Autophagy-targeted vaccine of LC3-LpqH DNA and its protective immunity in a murine model of tuberculosis.
Identifieur interne : 000F18 ( Main/Exploration ); précédent : 000F17; suivant : 000F19Autophagy-targeted vaccine of LC3-LpqH DNA and its protective immunity in a murine model of tuberculosis.
Auteurs : Dong Hu [République populaire de Chine] ; Jing Wu [République populaire de Chine] ; Rongbo Zhang [République populaire de Chine] ; Liping Chen [République populaire de Chine] ; Zhaoquan Chen [République populaire de Chine] ; Xuefeng Wang [République populaire de Chine] ; Lifa Xu [République populaire de Chine] ; Jian Xiao [République populaire de Chine] ; Fengyu Hu [République populaire de Chine] ; Changyou Wu [République populaire de Chine]Source :
- Vaccine [ 1873-2518 ] ; 2014.
Descripteurs français
- KwdFr :
- Animaux (MeSH), Anticorps antibactériens (sang), Autophagie (MeSH), Charge bactérienne (MeSH), Cytokines (immunologie), Femelle (MeSH), Lignée cellulaire (MeSH), Lipoprotéines (immunologie), Phagosomes (immunologie), Poumon (microbiologie), Protéines associées aux microtubules (immunologie), Protéines bactériennes (immunologie), Protéines de fusion recombinantes (immunologie), Rate (microbiologie), Souris (MeSH), Souris de lignée BALB C (MeSH), Tuberculose (prévention et contrôle), Vaccins antituberculeux (immunologie), Vaccins à ADN (immunologie).
- MESH :
- immunologie : Cytokines, Lipoprotéines, Phagosomes, Protéines associées aux microtubules, Protéines bactériennes, Protéines de fusion recombinantes, Vaccins antituberculeux, Vaccins à ADN.
- microbiologie : Poumon, Rate.
- prévention et contrôle : Tuberculose.
- sang : Anticorps antibactériens.
- Animaux, Autophagie, Charge bactérienne, Femelle, Lignée cellulaire, Souris, Souris de lignée BALB C.
English descriptors
- KwdEn :
- Animals (MeSH), Antibodies, Bacterial (blood), Autophagy (MeSH), Bacterial Load (MeSH), Bacterial Proteins (immunology), Cell Line (MeSH), Cytokines (immunology), Female (MeSH), Lipoproteins (immunology), Lung (microbiology), Mice (MeSH), Mice, Inbred BALB C (MeSH), Microtubule-Associated Proteins (immunology), Phagosomes (immunology), Recombinant Fusion Proteins (immunology), Spleen (microbiology), Tuberculosis (prevention & control), Tuberculosis Vaccines (immunology), Vaccines, DNA (immunology).
- MESH :
- chemical , blood : Antibodies, Bacterial.
- chemical , immunology : Bacterial Proteins, Cytokines, Lipoproteins, Microtubule-Associated Proteins, Recombinant Fusion Proteins, Tuberculosis Vaccines, Vaccines, DNA.
- immunology : Phagosomes.
- microbiology : Lung, Spleen.
- prevention & control : Tuberculosis.
- Animals, Autophagy, Bacterial Load, Cell Line, Female, Mice, Mice, Inbred BALB C.
Abstract
The development of more effective antituberculosis vaccines would contribute to the control of the global problem of infection with Mycobacterium tuberculosis (MTB). Recently, the highlighted importance of autophagy in the host immune response against MTB has attracted the attention of researchers. However, the vaccines targeted at autophagy remain to be developed. In this study, we report on an autophagy-targeted vaccine of 19kDa MTB lipoprotein (LpqH) DNA that harbors another gene coding microtubule-associated protein light chain-3(LC3), which transports LpqH to autophagosomes and displays enhanced protective efficacy against MTB. After the transfection of pCMV-LpqH DNA, a significant increase LC3 II was detected in RAW264.7 cells, which was similar to that observed with treatment with rapamycin, a reagent used to induce autophagy. To target autophagy, the gene coding LC3, as a marked protein of autophagosome, was linked to the lpqH gene to express an LC3-LpqH fused protein. Interestingly, LC3-LpqH fused protein was determined to be transported to an autophagosome, which was demonstrated by the co-localization of GFP-LC3 with LC3-LpqH at autophagosomes. Notably, the mice immunized with LC3-LpqH/Ag85B displayed decreased mycobacterial loads in the lungs and spleen when challenged with virulent MTB by intravenous infection, which was consistent with increased IgG2a in serum and IFN-γ and IL-2 produced by splenocyte. In conclusion, our study demonstrates that an LC3-LpqH DNA vaccine could have autophagy as its target, which contributes to the enhancement of the Th1 immune response and vaccine protective efficacy.
DOI: 10.1016/j.vaccine.2014.02.069
PubMed: 24631071
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Autophagy-targeted vaccine of LC3-LpqH DNA and its protective immunity in a murine model of tuberculosis.</title><author><name sortKey="Hu, Dong" sort="Hu, Dong" uniqKey="Hu D" first="Dong" last="Hu">Dong Hu</name><affiliation wicri:level="1"><nlm:affiliation>Department of Medical Immunology, Medical School, Anhui University of Science and Technology, Huainan, China; Institute of infection and immunology, Anhui University of Science and Technology, Huainan, China. Electronic address: austhudong@126.com.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Medical Immunology, Medical School, Anhui University of Science and Technology, Huainan, China; Institute of infection and immunology, Anhui University of Science and Technology, Huainan</wicri:regionArea><wicri:noRegion>Huainan</wicri:noRegion></affiliation></author><author><name sortKey="Wu, Jing" sort="Wu, Jing" uniqKey="Wu J" first="Jing" last="Wu">Jing Wu</name><affiliation wicri:level="1"><nlm:affiliation>Department of Medical Immunology, Medical School, Anhui University of Science and Technology, Huainan, China; Institute of infection and immunology, Anhui University of Science and Technology, Huainan, China. Electronic address: wujing8008@126.com.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Medical Immunology, Medical School, Anhui University of Science and Technology, Huainan, China; Institute of infection and immunology, Anhui University of Science and Technology, Huainan</wicri:regionArea><wicri:noRegion>Huainan</wicri:noRegion></affiliation></author><author><name sortKey="Zhang, Rongbo" sort="Zhang, Rongbo" uniqKey="Zhang R" first="Rongbo" last="Zhang">Rongbo Zhang</name><affiliation wicri:level="1"><nlm:affiliation>Department of Medical Immunology, Medical School, Anhui University of Science and Technology, Huainan, China; Institute of infection and immunology, Anhui University of Science and Technology, Huainan, China. Electronic address: lory456@126.com.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Medical Immunology, Medical School, Anhui University of Science and Technology, Huainan, China; Institute of infection and immunology, Anhui University of Science and Technology, Huainan</wicri:regionArea><wicri:noRegion>Huainan</wicri:noRegion></affiliation></author><author><name sortKey="Chen, Liping" sort="Chen, Liping" uniqKey="Chen L" first="Liping" last="Chen">Liping Chen</name><affiliation wicri:level="1"><nlm:affiliation>Department of Medical Immunology, Medical School, Anhui University of Science and Technology, Huainan, China; Institute of infection and immunology, Anhui University of Science and Technology, Huainan, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Medical Immunology, Medical School, Anhui University of Science and Technology, Huainan, China; Institute of infection and immunology, Anhui University of Science and Technology, Huainan</wicri:regionArea><wicri:noRegion>Huainan</wicri:noRegion></affiliation></author><author><name sortKey="Chen, Zhaoquan" sort="Chen, Zhaoquan" uniqKey="Chen Z" first="Zhaoquan" last="Chen">Zhaoquan Chen</name><affiliation wicri:level="1"><nlm:affiliation>Institute of infection and immunology, Anhui University of Science and Technology, Huainan, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of infection and immunology, Anhui University of Science and Technology, Huainan</wicri:regionArea><wicri:noRegion>Huainan</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Xuefeng" sort="Wang, Xuefeng" uniqKey="Wang X" first="Xuefeng" last="Wang">Xuefeng Wang</name><affiliation wicri:level="1"><nlm:affiliation>Department of Medical Immunology, Medical School, Anhui University of Science and Technology, Huainan, China; Institute of infection and immunology, Anhui University of Science and Technology, Huainan, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Medical Immunology, Medical School, Anhui University of Science and Technology, Huainan, China; Institute of infection and immunology, Anhui University of Science and Technology, Huainan</wicri:regionArea><wicri:noRegion>Huainan</wicri:noRegion></affiliation></author><author><name sortKey="Xu, Lifa" sort="Xu, Lifa" uniqKey="Xu L" first="Lifa" last="Xu">Lifa Xu</name><affiliation wicri:level="1"><nlm:affiliation>Department of Medical Immunology, Medical School, Anhui University of Science and Technology, Huainan, China; Institute of infection and immunology, Anhui University of Science and Technology, Huainan, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Medical Immunology, Medical School, Anhui University of Science and Technology, Huainan, China; Institute of infection and immunology, Anhui University of Science and Technology, Huainan</wicri:regionArea><wicri:noRegion>Huainan</wicri:noRegion></affiliation></author><author><name sortKey="Xiao, Jian" sort="Xiao, Jian" uniqKey="Xiao J" first="Jian" last="Xiao">Jian Xiao</name><affiliation wicri:level="1"><nlm:affiliation>School of Pharmacy, Wenzhou Medical College, Wenzhou, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>School of Pharmacy, Wenzhou Medical College, Wenzhou</wicri:regionArea><wicri:noRegion>Wenzhou</wicri:noRegion></affiliation></author><author><name sortKey="Hu, Fengyu" sort="Hu, Fengyu" uniqKey="Hu F" first="Fengyu" last="Hu">Fengyu Hu</name><affiliation wicri:level="3"><nlm:affiliation>Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Wu, Changyou" sort="Wu, Changyou" uniqKey="Wu C" first="Changyou" last="Wu">Changyou Wu</name><affiliation wicri:level="3"><nlm:affiliation>Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2014">2014</date><idno type="RBID">pubmed:24631071</idno><idno type="pmid">24631071</idno><idno type="doi">10.1016/j.vaccine.2014.02.069</idno><idno type="wicri:Area/Main/Corpus">000E82</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000E82</idno><idno type="wicri:Area/Main/Curation">000E82</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000E82</idno><idno type="wicri:Area/Main/Exploration">000E82</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Autophagy-targeted vaccine of LC3-LpqH DNA and its protective immunity in a murine model of tuberculosis.</title><author><name sortKey="Hu, Dong" sort="Hu, Dong" uniqKey="Hu D" first="Dong" last="Hu">Dong Hu</name><affiliation wicri:level="1"><nlm:affiliation>Department of Medical Immunology, Medical School, Anhui University of Science and Technology, Huainan, China; Institute of infection and immunology, Anhui University of Science and Technology, Huainan, China. Electronic address: austhudong@126.com.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Medical Immunology, Medical School, Anhui University of Science and Technology, Huainan, China; Institute of infection and immunology, Anhui University of Science and Technology, Huainan</wicri:regionArea><wicri:noRegion>Huainan</wicri:noRegion></affiliation></author><author><name sortKey="Wu, Jing" sort="Wu, Jing" uniqKey="Wu J" first="Jing" last="Wu">Jing Wu</name><affiliation wicri:level="1"><nlm:affiliation>Department of Medical Immunology, Medical School, Anhui University of Science and Technology, Huainan, China; Institute of infection and immunology, Anhui University of Science and Technology, Huainan, China. Electronic address: wujing8008@126.com.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Medical Immunology, Medical School, Anhui University of Science and Technology, Huainan, China; Institute of infection and immunology, Anhui University of Science and Technology, Huainan</wicri:regionArea><wicri:noRegion>Huainan</wicri:noRegion></affiliation></author><author><name sortKey="Zhang, Rongbo" sort="Zhang, Rongbo" uniqKey="Zhang R" first="Rongbo" last="Zhang">Rongbo Zhang</name><affiliation wicri:level="1"><nlm:affiliation>Department of Medical Immunology, Medical School, Anhui University of Science and Technology, Huainan, China; Institute of infection and immunology, Anhui University of Science and Technology, Huainan, China. Electronic address: lory456@126.com.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Medical Immunology, Medical School, Anhui University of Science and Technology, Huainan, China; Institute of infection and immunology, Anhui University of Science and Technology, Huainan</wicri:regionArea><wicri:noRegion>Huainan</wicri:noRegion></affiliation></author><author><name sortKey="Chen, Liping" sort="Chen, Liping" uniqKey="Chen L" first="Liping" last="Chen">Liping Chen</name><affiliation wicri:level="1"><nlm:affiliation>Department of Medical Immunology, Medical School, Anhui University of Science and Technology, Huainan, China; Institute of infection and immunology, Anhui University of Science and Technology, Huainan, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Medical Immunology, Medical School, Anhui University of Science and Technology, Huainan, China; Institute of infection and immunology, Anhui University of Science and Technology, Huainan</wicri:regionArea><wicri:noRegion>Huainan</wicri:noRegion></affiliation></author><author><name sortKey="Chen, Zhaoquan" sort="Chen, Zhaoquan" uniqKey="Chen Z" first="Zhaoquan" last="Chen">Zhaoquan Chen</name><affiliation wicri:level="1"><nlm:affiliation>Institute of infection and immunology, Anhui University of Science and Technology, Huainan, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of infection and immunology, Anhui University of Science and Technology, Huainan</wicri:regionArea><wicri:noRegion>Huainan</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Xuefeng" sort="Wang, Xuefeng" uniqKey="Wang X" first="Xuefeng" last="Wang">Xuefeng Wang</name><affiliation wicri:level="1"><nlm:affiliation>Department of Medical Immunology, Medical School, Anhui University of Science and Technology, Huainan, China; Institute of infection and immunology, Anhui University of Science and Technology, Huainan, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Medical Immunology, Medical School, Anhui University of Science and Technology, Huainan, China; Institute of infection and immunology, Anhui University of Science and Technology, Huainan</wicri:regionArea><wicri:noRegion>Huainan</wicri:noRegion></affiliation></author><author><name sortKey="Xu, Lifa" sort="Xu, Lifa" uniqKey="Xu L" first="Lifa" last="Xu">Lifa Xu</name><affiliation wicri:level="1"><nlm:affiliation>Department of Medical Immunology, Medical School, Anhui University of Science and Technology, Huainan, China; Institute of infection and immunology, Anhui University of Science and Technology, Huainan, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Medical Immunology, Medical School, Anhui University of Science and Technology, Huainan, China; Institute of infection and immunology, Anhui University of Science and Technology, Huainan</wicri:regionArea><wicri:noRegion>Huainan</wicri:noRegion></affiliation></author><author><name sortKey="Xiao, Jian" sort="Xiao, Jian" uniqKey="Xiao J" first="Jian" last="Xiao">Jian Xiao</name><affiliation wicri:level="1"><nlm:affiliation>School of Pharmacy, Wenzhou Medical College, Wenzhou, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>School of Pharmacy, Wenzhou Medical College, Wenzhou</wicri:regionArea><wicri:noRegion>Wenzhou</wicri:noRegion></affiliation></author><author><name sortKey="Hu, Fengyu" sort="Hu, Fengyu" uniqKey="Hu F" first="Fengyu" last="Hu">Fengyu Hu</name><affiliation wicri:level="3"><nlm:affiliation>Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Wu, Changyou" sort="Wu, Changyou" uniqKey="Wu C" first="Changyou" last="Wu">Changyou Wu</name><affiliation wicri:level="3"><nlm:affiliation>Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author></analytic><series><title level="j">Vaccine</title><idno type="eISSN">1873-2518</idno><imprint><date when="2014" type="published">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals (MeSH)</term><term>Antibodies, Bacterial (blood)</term><term>Autophagy (MeSH)</term><term>Bacterial Load (MeSH)</term><term>Bacterial Proteins (immunology)</term><term>Cell Line (MeSH)</term><term>Cytokines (immunology)</term><term>Female (MeSH)</term><term>Lipoproteins (immunology)</term><term>Lung (microbiology)</term><term>Mice (MeSH)</term><term>Mice, Inbred BALB C (MeSH)</term><term>Microtubule-Associated Proteins (immunology)</term><term>Phagosomes (immunology)</term><term>Recombinant Fusion Proteins (immunology)</term><term>Spleen (microbiology)</term><term>Tuberculosis (prevention & control)</term><term>Tuberculosis Vaccines (immunology)</term><term>Vaccines, DNA (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux (MeSH)</term><term>Anticorps antibactériens (sang)</term><term>Autophagie (MeSH)</term><term>Charge bactérienne (MeSH)</term><term>Cytokines (immunologie)</term><term>Femelle (MeSH)</term><term>Lignée cellulaire (MeSH)</term><term>Lipoprotéines (immunologie)</term><term>Phagosomes (immunologie)</term><term>Poumon (microbiologie)</term><term>Protéines associées aux microtubules (immunologie)</term><term>Protéines bactériennes (immunologie)</term><term>Protéines de fusion recombinantes (immunologie)</term><term>Rate (microbiologie)</term><term>Souris (MeSH)</term><term>Souris de lignée BALB C (MeSH)</term><term>Tuberculose (prévention et contrôle)</term><term>Vaccins antituberculeux (immunologie)</term><term>Vaccins à ADN (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Bacterial</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Bacterial Proteins</term><term>Cytokines</term><term>Lipoproteins</term><term>Microtubule-Associated Proteins</term><term>Recombinant Fusion Proteins</term><term>Tuberculosis Vaccines</term><term>Vaccines, DNA</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Cytokines</term><term>Lipoprotéines</term><term>Phagosomes</term><term>Protéines associées aux microtubules</term><term>Protéines bactériennes</term><term>Protéines de fusion recombinantes</term><term>Vaccins antituberculeux</term><term>Vaccins à ADN</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Phagosomes</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Poumon</term><term>Rate</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Lung</term><term>Spleen</term></keywords><keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Tuberculosis</term></keywords><keywords scheme="MESH" qualifier="prévention et contrôle" xml:lang="fr"><term>Tuberculose</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Anticorps antibactériens</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Autophagy</term><term>Bacterial Load</term><term>Cell Line</term><term>Female</term><term>Mice</term><term>Mice, Inbred BALB C</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Autophagie</term><term>Charge bactérienne</term><term>Femelle</term><term>Lignée cellulaire</term><term>Souris</term><term>Souris de lignée BALB C</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The development of more effective antituberculosis vaccines would contribute to the control of the global problem of infection with Mycobacterium tuberculosis (MTB). Recently, the highlighted importance of autophagy in the host immune response against MTB has attracted the attention of researchers. However, the vaccines targeted at autophagy remain to be developed. In this study, we report on an autophagy-targeted vaccine of 19kDa MTB lipoprotein (LpqH) DNA that harbors another gene coding microtubule-associated protein light chain-3(LC3), which transports LpqH to autophagosomes and displays enhanced protective efficacy against MTB. After the transfection of pCMV-LpqH DNA, a significant increase LC3 II was detected in RAW264.7 cells, which was similar to that observed with treatment with rapamycin, a reagent used to induce autophagy. To target autophagy, the gene coding LC3, as a marked protein of autophagosome, was linked to the lpqH gene to express an LC3-LpqH fused protein. Interestingly, LC3-LpqH fused protein was determined to be transported to an autophagosome, which was demonstrated by the co-localization of GFP-LC3 with LC3-LpqH at autophagosomes. Notably, the mice immunized with LC3-LpqH/Ag85B displayed decreased mycobacterial loads in the lungs and spleen when challenged with virulent MTB by intravenous infection, which was consistent with increased IgG2a in serum and IFN-γ and IL-2 produced by splenocyte. In conclusion, our study demonstrates that an LC3-LpqH DNA vaccine could have autophagy as its target, which contributes to the enhancement of the Th1 immune response and vaccine protective efficacy. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">24631071</PMID><DateCompleted><Year>2014</Year><Month>10</Month><Day>03</Day></DateCompleted><DateRevised><Year>2014</Year><Month>04</Month><Day>07</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1873-2518</ISSN><JournalIssue CitedMedium="Internet"><Volume>32</Volume><Issue>20</Issue><PubDate><Year>2014</Year><Month>Apr</Month><Day>25</Day></PubDate></JournalIssue><Title>Vaccine</Title><ISOAbbreviation>Vaccine</ISOAbbreviation></Journal><ArticleTitle>Autophagy-targeted vaccine of LC3-LpqH DNA and its protective immunity in a murine model of tuberculosis.</ArticleTitle><Pagination><MedlinePgn>2308-14</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.vaccine.2014.02.069</ELocationID><ELocationID EIdType="pii" ValidYN="Y">S0264-410X(14)00296-5</ELocationID><Abstract><AbstractText>The development of more effective antituberculosis vaccines would contribute to the control of the global problem of infection with Mycobacterium tuberculosis (MTB). Recently, the highlighted importance of autophagy in the host immune response against MTB has attracted the attention of researchers. However, the vaccines targeted at autophagy remain to be developed. In this study, we report on an autophagy-targeted vaccine of 19kDa MTB lipoprotein (LpqH) DNA that harbors another gene coding microtubule-associated protein light chain-3(LC3), which transports LpqH to autophagosomes and displays enhanced protective efficacy against MTB. After the transfection of pCMV-LpqH DNA, a significant increase LC3 II was detected in RAW264.7 cells, which was similar to that observed with treatment with rapamycin, a reagent used to induce autophagy. To target autophagy, the gene coding LC3, as a marked protein of autophagosome, was linked to the lpqH gene to express an LC3-LpqH fused protein. Interestingly, LC3-LpqH fused protein was determined to be transported to an autophagosome, which was demonstrated by the co-localization of GFP-LC3 with LC3-LpqH at autophagosomes. Notably, the mice immunized with LC3-LpqH/Ag85B displayed decreased mycobacterial loads in the lungs and spleen when challenged with virulent MTB by intravenous infection, which was consistent with increased IgG2a in serum and IFN-γ and IL-2 produced by splenocyte. In conclusion, our study demonstrates that an LC3-LpqH DNA vaccine could have autophagy as its target, which contributes to the enhancement of the Th1 immune response and vaccine protective efficacy. </AbstractText><CopyrightInformation>Copyright © 2014 Elsevier Ltd. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Hu</LastName><ForeName>Dong</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Department of Medical Immunology, Medical School, Anhui University of Science and Technology, Huainan, China; Institute of infection and immunology, Anhui University of Science and Technology, Huainan, China. Electronic address: austhudong@126.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wu</LastName><ForeName>Jing</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Department of Medical Immunology, Medical School, Anhui University of Science and Technology, Huainan, China; Institute of infection and immunology, Anhui University of Science and Technology, Huainan, China. Electronic address: wujing8008@126.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Rongbo</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Department of Medical Immunology, Medical School, Anhui University of Science and Technology, Huainan, China; Institute of infection and immunology, Anhui University of Science and Technology, Huainan, China. Electronic address: lory456@126.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Liping</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Department of Medical Immunology, Medical School, Anhui University of Science and Technology, Huainan, China; Institute of infection and immunology, Anhui University of Science and Technology, Huainan, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Zhaoquan</ForeName><Initials>Z</Initials><AffiliationInfo><Affiliation>Institute of infection and immunology, Anhui University of Science and Technology, Huainan, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Xuefeng</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>Department of Medical Immunology, Medical School, Anhui University of Science and Technology, Huainan, China; Institute of infection and immunology, Anhui University of Science and Technology, Huainan, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xu</LastName><ForeName>Lifa</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Department of Medical Immunology, Medical School, Anhui University of Science and Technology, Huainan, China; Institute of infection and immunology, Anhui University of Science and Technology, Huainan, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xiao</LastName><ForeName>Jian</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>School of Pharmacy, Wenzhou Medical College, Wenzhou, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hu</LastName><ForeName>Fengyu</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wu</LastName><ForeName>Changyou</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2014</Year><Month>03</Month><Day>12</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Vaccine</MedlineTA><NlmUniqueID>8406899</NlmUniqueID><ISSNLinking>0264-410X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000907">Antibodies, Bacterial</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D001426">Bacterial Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D016207">Cytokines</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D008074">Lipoproteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D008869">Microtubule-Associated Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011993">Recombinant Fusion Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D032581">Tuberculosis Vaccines</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D019444">Vaccines, DNA</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000907" MajorTopicYN="N">Antibodies, Bacterial</DescriptorName><QualifierName UI="Q000097" MajorTopicYN="N">blood</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001343" MajorTopicYN="Y">Autophagy</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D058491" MajorTopicYN="N">Bacterial Load</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001426" MajorTopicYN="N">Bacterial Proteins</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002460" MajorTopicYN="N">Cell Line</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016207" MajorTopicYN="N">Cytokines</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005260" MajorTopicYN="N">Female</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008074" MajorTopicYN="N">Lipoproteins</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008168" MajorTopicYN="N">Lung</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D051379" MajorTopicYN="N">Mice</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008807" MajorTopicYN="N">Mice, Inbred BALB C</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008869" MajorTopicYN="N">Microtubule-Associated Proteins</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010588" MajorTopicYN="N">Phagosomes</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011993" MajorTopicYN="N">Recombinant Fusion Proteins</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013154" MajorTopicYN="N">Spleen</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014376" MajorTopicYN="N">Tuberculosis</DescriptorName><QualifierName UI="Q000517" MajorTopicYN="Y">prevention & control</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032581" MajorTopicYN="N">Tuberculosis Vaccines</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019444" MajorTopicYN="N">Vaccines, DNA</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Ag85B</Keyword><Keyword MajorTopicYN="N">Autophagy</Keyword><Keyword MajorTopicYN="N">LC3</Keyword><Keyword MajorTopicYN="N">LpqH</Keyword><Keyword MajorTopicYN="N">Mycobacterium tuberculosis</Keyword><Keyword MajorTopicYN="N">Protective immunity</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2013</Year><Month>06</Month><Day>21</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2014</Year><Month>02</Month><Day>07</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2014</Year><Month>02</Month><Day>25</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2014</Year><Month>3</Month><Day>18</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2014</Year><Month>3</Month><Day>19</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2014</Year><Month>10</Month><Day>4</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">24631071</ArticleId><ArticleId IdType="pii">S0264-410X(14)00296-5</ArticleId><ArticleId IdType="doi">10.1016/j.vaccine.2014.02.069</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country><region><li>Guangdong</li></region><settlement><li>Jiangmen</li></settlement></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Hu, Dong" sort="Hu, Dong" uniqKey="Hu D" first="Dong" last="Hu">Dong Hu</name></noRegion><name sortKey="Chen, Liping" sort="Chen, Liping" uniqKey="Chen L" first="Liping" last="Chen">Liping Chen</name><name sortKey="Chen, Zhaoquan" sort="Chen, Zhaoquan" uniqKey="Chen Z" first="Zhaoquan" last="Chen">Zhaoquan Chen</name><name sortKey="Hu, Fengyu" sort="Hu, Fengyu" uniqKey="Hu F" first="Fengyu" last="Hu">Fengyu Hu</name><name sortKey="Wang, Xuefeng" sort="Wang, Xuefeng" uniqKey="Wang X" first="Xuefeng" last="Wang">Xuefeng Wang</name><name sortKey="Wu, Changyou" sort="Wu, Changyou" uniqKey="Wu C" first="Changyou" last="Wu">Changyou Wu</name><name sortKey="Wu, Jing" sort="Wu, Jing" uniqKey="Wu J" first="Jing" last="Wu">Jing Wu</name><name sortKey="Xiao, Jian" sort="Xiao, Jian" uniqKey="Xiao J" first="Jian" last="Xiao">Jian Xiao</name><name sortKey="Xu, Lifa" sort="Xu, Lifa" uniqKey="Xu L" first="Lifa" last="Xu">Lifa Xu</name><name sortKey="Zhang, Rongbo" sort="Zhang, Rongbo" uniqKey="Zhang R" first="Rongbo" last="Zhang">Rongbo Zhang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/RapamycinFungusV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000F18 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000F18 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= RapamycinFungusV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:24631071
|texte= Autophagy-targeted vaccine of LC3-LpqH DNA and its protective immunity in a murine model of tuberculosis.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:24631071" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a RapamycinFungusV1
| This area was generated with Dilib version V0.6.38. |  |